Protein_Domain

Part:BBa_M50009

Designed by: Amanda Urke   Group: Stanford BIOE44 - S11   (2016-11-29)


mRuby3 fluorescent protein

mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in fluorescent resonance energy transfer (FRET) analysis as an acceptor fluorophore when paired with another compatible fluorescent protein, like a GFP. Emission and absorption spectra must be analyzed for each fluorophore member in the FRET pair to ensure proper overlap and optimal FRET efficiency.


Team Bielefeld-CeBiTec improved this part. The improved version BBa_KK3900021 can be found under the following link: https://parts.igem.org/Part:BBa_K3900021


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


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Parameters
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